Mladic, indicted for genocide and crimes against humanity, was transferred from the detention centre of the Belgrade special court to the Netherlands after the Justice Minister signed an order for his extradition following the court's rejection of the appeal by the Mladic defence team against his handover.
The court ruled last Friday that Mladic was fit to stand trial in The Hague.
Mladic was captured in the village of Lazarevo, 80 kilometres northeast of Belgrade, last Thursday after 16 years on the run. He was indicted by the ICTY in 1995 for genocide, crimes against humanity and war crimes his troops committed during the 1992-1995 war in Bosnia and Herzegovina.
Mladic and former Bosnian Serb political leader Radovan Karadzic, who is on trial in The Hague, are held responsible for the murder of 8,000 Bosnian Muslims in Srebrenica in 1995 and for the 43-month-long siege of Sarajevo.