As the US government has stated several times, there is no such agreement, the US Embassy in Podgorica said in a statement.
Montenegrin daily Republika wrote that Holbrooke had signed an agreement with Karadzic guaranteeing him on behalf of the US government absolute security provided he withdrew from public life.
The newspaper published a copy of a document tentatively called "Commitments", which it said had been signed in June 1996.
Karadzic's wife Ljiljana Zelen-Karadzic reportedly confirmed the existence of the agreement in a conversation with a Republika journalist in Pale, just east of Sarajevo, on March 7, 2004.
Many US officials have rejected the existence of such an agreement with Karadzic, who was indicted by the Hague tribunal in 1996 for the gravest of war crimes, including genocide.