"Not guilty, Your Honour," Bralo said at his second appearance before judges as each count of the indictment was read to him.
Bralo, age 37, surrendered to NATO-led peacekeeping forces in Bosnia on November 10. He did not enter a plea at his initial appearance on November 15, but asked the judges for permission to do so within a prescribed period of 30 days.
The accused was accompanied by his provisional defence counsel, Virginia Lindsay, who also represented him at his initial appearance.
Bralo was a member of a Bosnian Croat special unit known as the Jokers. He is accused of murdering three Muslim prisoners and repeatedly brutally raping a detained Muslim woman, identified in the indictment as Witness A, in the unit's headquarters in Nadioci near Vitez in mid-May 1993.
Bralo was also cited in other cases of war crimes committed in the Lasva valley, notably in the trials of Tihomir Blaskic, Dario Blaskic, Dario Kordic and Mario Cerkez in connection with an atrocity committed by Croat forces in the predominantly Muslim village of Ahmici.
Bralo's superior, the Jokers' commander Anto Furundzija, was sentenced by the tribunal in 1998 to 10 years in prison for failing to prevent the rape of Witness A.
Asked by Judge Amin El Mahdi of Egypt about his health and prison conditions, Bralo said he was satisfied with prison conditions and that he had no health problems.
The judge said he would request that Registrar Hans Holthis set a date for the start of the trial.
Chief Prosecutor Carla del Ponte demanded in her address at NATO headquarters in Brussels on November 3 that Croatian authorities arrest Bralo. After he surrendered to the Stabilisation Force (SFOR) in Bosnia a week later, del Ponte commended Croatia for "cross-border cooperation" in resolving the case in her report to the UN Security Council on November 23.
The indictment against Bralo is dated November 10, 1995, but was unsealed in October this year.