Croatian President Stjepan Mesic also paid his respects by laying a wreath and lighting a candle.
On behalf of the Croatian parliament, wreaths were laid by a delegation led by parliament deputy speaker Darko Milinovic, while War Veterans Minister Jadranka Kosor laid a wreath on the government's behalf.
Wreaths were also laid by representatives of the Armed Forces, local officials, war veterans associations, and political parties.
Mass for all the victims was said by Dubrovnik bishop Zelimir Puljic.
At least 1,500 soldiers and civilians were killed in the defence of Vukovar. Thousands of people were wounded, more than 20,000 Croats and other non-Serbs were exiled, thousands of soldiers and civilians were detained in Serb concentration camps, while about 500 are still listed as missing.
This year Remembrance Day for the Vukovar Victims of 1991 is marked under the motto "Sound All the Bells".
Parliament decided in 1998 that November 18 would be commemorated as Remembrance Day for the Vukovar Victims of 1991.