Skupina 1:
1. Sanya Richards (SAD) 50.05 Q
2. Amy Mbacke Thiam (Sen) 50.83 Q
3. Natalia Antjukh (Rus) 50.99
4. Nawal El Jack (Sud) 51.85
5. Tiandra Ponteen (SKN) 51.88
6. Shericka Williams (Jam) 52.44
7. Donna Fraser (VB) 52.48
8. Anna Kozak (Blr) 52.73
Skupina 2:
1. Svetlana Pospelova (Rus) 50.34 Q
2. DeeDee Trotter (SAD) 50.73 Q
3. Christine Amertil (Bah) 51.03
4. Christine Ohuruogu (VB) 51.43
5. Lorraine Fenton (Jam) 51.48
6. Kaltouma Nadjina (Čad) 52.07
7. Fatou Bintou Fall (Sen) 52.35
8. Anna Guzowska (Polj) 52.45
Skupina 3:
1. Tonique Darling (Bah) 49.69 Q
2. Ana Guevara (Mek) 50.33 Q
3. Olesja Zykina (Rus) 50.73
4. Monique Henderson (SAD) 50.73
5. Ilona Usovich (Blr) 50.96
6. Lee McConnell (VB) 51.15
7. Lucimar Teodoro (Bra) 51.98
8. Karen Shinkins (Irs) 52.17